By Z. Nawaz, et. al.
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37 38 Advances in Chemical Engineering Fig. 18. Comparison between experimental and calculated values of the amount of asphaltene precipitation vs. , 2009) using SAFT EoS and WinProp. Application of Chebyshev Polynomials to Calculate Density and Fugacity Using SAFT Equation of State to Predict Asphaltene Precipitation Conditions Fig. 19. Comparison between experimental and calculated values of the amount of asphaltene precipitation vs. , 2009) using SAFT EoS and WinProp. 39 40 Advances in Chemical Engineering 5.
3b is the second episode OGIs. 3 b as excited at 365 nm (internal light source). The OGI emits light yellow fluorescence. b a c Fig. 3. 1 m; and c the fluorescence image of b under excited at 365 nm internal light source in the microscope. The scale in a is 20 m, and in b and c 10 m. 3. 4 shows the schematic diagram of the experimental setup (Yang, 2009a, 2009b), which includes seven parts: (1) an inverted fluorescence microscope (IMF); (2) a reflecting microscope objective (RMO); (3) a 3D adaptor; (4) a micro lens; (5) a fiber cable; (6) a spectrometer and (7) a computer.
The third is the expensive DUV microscope, its price is 9-20 times of a common fluorescence microscope. This will greatly limit it using. The fourth is that for effective collecting fluorescence an oil immersed objective had to be used, which is trouble to clean. The above experimental system is so expensive and one has to rebuild the microscope. Is it possible to replace the DUV microscope with a common microscope to lower cost and still detect UV-VIS fluorescence spectra of single OGIs? Such kind of micro-spectroscopy system to meet this aim was established (Yang, 2009a, 2009b, 2011).
Advances in Chemical Engineering by Z. Nawaz, et. al.